Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP signal detection system

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Abstract

We report the development of a simple and rapid PCR assay for quantitation of the cytomegalovirus (CMV) DNA load in polymorphonuclear leukocytes. Using this system, a very good correlation was found between a high number of CMV copies in the blood and the presence of CMV disease in subjects with AIDS.

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Boivin, G., Handfield, J., Murray, G., Toma, E., Lalonde, R., Lazar, J. G., & Bergeron, M. G. (1997). Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP signal detection system. Journal of Clinical Microbiology, 35(2), 525–526. https://doi.org/10.1128/jcm.35.2.525-526.1997

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