Antioxidant activity of isolated phytoconstituents from the leaves of Hyptis suaveolens (L.) Poit

Abstract

Hyptis suaveolens (L.) Poit (Family Lamiaceae) is a medicinal plant used traditionally for antifertility, anti-inflammatory and antiplasmodial properties. Therefore, this study was designed to isolate bioactive compounds and to evaluate the antioxidant activity of isolated compounds from H. suaveolens by silica gel Column Chromatographic separation method and gallic acid (10 mg) was mainly isolated. Among them, the isolated compound was structurally characterized by physicochemical determination and spectroscopic techniques such as UV, FTIR and 1H-NMR by comparing with the reported data. Moreover, the essential oil was extracted from the fresh leaves of H. suaveolens by the hydro-distillation method and analyzed by GC-MS. It was found that carophyllene, sabinene, and naphthalene were predominant compounds in the essential oil of H. suaveolens. The antioxidant activity of isolated compounds and essential oil was determined by DPPH free radical scavenging assay method. The results suggest that the major compounds gallic acid from H. suaveolens possess effective antioxidant activity.