Sensitivity improvement of an impedimetric immunosensor using functionalized iron oxide nanoparticles

Abstract

This work has explored the development of impedimetric immunosensors based on magnetic iron nanoparticles (IrNP) functionalized with streptavidin to which a biotinylated FAB part of the antibody has been bound using a biotin-streptavidin interaction. SPR analysis shows a deviation on the measured (angle) during antigen-antibody recognition whereas label free detection using by EIS allows us to monitor variation of polarization resistance. Before detection, layers were analyzed by FTIR and AFM. Compared to immobilization of antibody on bare gold surface using aminodecanethiol SAM, antibody immobilization on nanoparticles permitted to reach lower detection limit: 500pg/ml instead of 1ng/ml to in the case of EIS and 300ng/ml instead of 4.5 g/ml in the case of SPR. Thus, it permitted to improve the sensitivity: from 257.3 ω ̇ cm2 ̇ μg-1 ̇ ml to 1871 ω ̇ cm2 ̇ μg-1 ̇ ml in the case of EIS and from 0.003° μg-1 ̇ ml to 0.094° μg -1 ̇ ml in the case of SPR. Copyright © 2009 Imen Hafaid et al.