A simple and quick technique for establishing axenic cultures of the centric diatom Coscinodiscus wailesii Gran

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Abstract

We devised a simple and quick technique for establishing axenic cultures of the centric diatom Coscinodiscus wailesii Gran by washing the cells with a sieve and soaking them in antibiotic media.

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Nagai, S., Imai, I., & Manabe, T. (1998). A simple and quick technique for establishing axenic cultures of the centric diatom Coscinodiscus wailesii Gran. Journal of Plankton Research, 20(7), 1417–1420. https://doi.org/10.1093/plankt/20.7.1417

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