Development of a rapid and reversible photocrosslinking of RNA.

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Abstract

We describe a novel reversible interstrand photocrosslinking reaction of RNA. In this system, a modified oligodeoxynucleotide (ODN) containing 3-cyanovinylcarbazole nucleoside ((CNV)K) reacts by photoirradiation at 366 nm for 1 s with pyrimidine residue of a complementary template RNA to yield a crosslinked product in 94% yield.

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Yoshimura, Y., Ohtake, T., Okada, H., & Fujimoto, K. (2009). Development of a rapid and reversible photocrosslinking of RNA. Nucleic Acids Symposium Series (2004), (53), 197–198. https://doi.org/10.1093/nass/nrp099

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