The phylogenetic study of Escherichia coli strains isolated from clinical cases

Abstract

Urinary tract infection (UTI) is one of the common infectious diseases. Escherichia coli is one of the most important cause of UTI. The purpose of this research was to determine the frequency of some phylogenetic genes in E. coli strains isolated from patients with UTI. E. coli strains were recovered from urine culture of suspect patients with UTI and biochemical and bacterial tests have been utilized to identify the isolated bacteria. Detection of phylogenetic genes were done through Multiplex PCR. Approximately 94%, 92%, 82% and 72% of E. coli strains contained ChuA1, YjaA, TspE4C and arp genes respectively. Results indicate that PCR based phylogenetic molecular method is a simple and quick method for typing of our uropathogenic E. coli.