Evaluation of in-vitro antioxidant potential and in-vivo hepatoprotective activity of root extract of Quercus oblongata D. DON

Abstract

Objective: The main potential target is attempt to investigated evaluation of in-vitro antioxidant potential and in-vivo hepatoprotective activity of root extract of Quercus oblongata D. DON belonging to family fagaceae. Material & Methods: The root of plant was extracted by different solvents like n-hexane (NHEQO), Chloroform (CEQO), Ethyl acetate (EAQO) Hydroethanolic (HEEQO) and Ethanol (EEQO). The antioxidant activity (AA) was determined by the possible four complementary test assay methods namely total phenolic content, total flavonoids content, Inhibition of  2,2 diphenyl -1 picrylhydrazyl (DPPH) radicals and ABTS (2-2’- azinobis) radical scavenging activity or quenching activity, in the hepatoprotective experimental  animal albino wistar rats (120-180gm) were divided into 6 group, each group content 5, Group I: Received distilled water (5ml/kg. p.o) once daily, and served as normal control. Group II: Received paracetamol suspension (640 mg/kg suspended in 1% methyl cellulose; orally as toxin control. Group III: Received standard drug Silymarin (25 mg/kg. p.o.) + paracetamol suspension (640 mg/kg suspended in 1% methyl cellulose; orally once daily Group IV, V, VI administered HEECB at different doses300, 400, 500 mg/kg orally + paracetamol suspension (640 mg/kg suspended in 1% methyl cellulose; for 21 days. And collect blood from experimental animals by retrorbital puncture for estimation of biochemical parameters and other parameter also evaluate like physical histological changes in livers of rats. Results: Experimental finding reveal that Paracetamol produce significant change in physical (increase liver weight) biochemical (increase alkaline phosphate, serum glutamic oxalacetic transaminase, serum glutamic pyuruvic transaminase, total protein, total bilirubin, direct bilirubin and decrease the level of total protein and albumin) histological (damage to hepatocyte) and in liver parameters. Pretreatment with extract significantly minimization of physical, biochemical, histological and functional change induced by Paracetamol in liver. Conclusion: Experimental data and analysis of different parameter declare that hydroethanolic extract of Quercus oblongata could be a useful hepatoprotective agents and antioxidant potential. Keywords: Clematis buchananiana, paracetamol, hepatoprotective, alkaline phosphate, serum glutamic oxalacetic transaminase, serum glutamic pyuruvic transaminase.