Anti-oxidant, DNA-damage protection and anti-cancer properties of n-butanol extract of the endemic Perralderia coronopifolia

Abstract

This study was designed to evaluate in vitro the total phenolic, flavonoid content, anti-oxidant activity, oxidative DNA-damage protection and anticancer activity of n-butanol extract from Perralderia coronopifolia, endemic plant. DNA protection capacity was performed using 46966 plasmid DNA against UV-photolysis of H2O2-induced oxidative damage. The anti-proliferative effects of extract were performed on HeLa and C6 cells. Experimental results showed that extract had convenient number of phenolic and flavonoids. Furthermore, it provided strong anti-oxidant, reducing power, hydrogen peroxide and higher DPPH? scavenging ability with IC50= 7.02 ± 0.02 µg/ mL. As it shown an oxidative plasmid DNA-damage inhibition against UVphotolysis of H2O2, an anti-cancer activity at higher concentrations in cells. The data obtained that P. coronopifolia extract could be useful in human protection against infection and degenerative illness.