MSX2 overexpression inhibits gemcitabine-induced caspase-3 activity in pancreatic cancer cells

Abstract

Aim: To evaluate the effect of MSX2 on gemcitabine-induced caspase-3 activation in pancreatic cancer cell line Panc-1. Methods: Using V5-tagged MSX2 expression vector, stable transfectant of MSX2 was generated from Panc-1 cells (P×14 cells). Cell viability under gemcitabine administration was determined by MTT assay relative to control cell line (empty-vector transfected Panc-1 cells; P-3EV cells). Hoechst staining was used for the detection of apoptotic cell. Activation of caspase-3 was assessed using Western blotting analysis and direct measurement of caspase-3 specific activities. Results: MSX2 overexpression in Panc-1 cells resulted in decreased gemcitabine-induced caspase-3 activation and increased cell viability under gemcitabine treatment in P×14 cells. Conclusion: MSX2 exerts repressive effects on gemcitabine-induced apoptotic pathway. This novel apoptosis-regulating function of MSX2 may provide a new therapeutic target for pancreatic cancer. © 2005 The WJG Press and Elsevier Inc. All rights reserved.