Gret1 retrotransposon and VvmybA1 gene sequences in somatic mutants of new table grape varieties a Brasila and a Black Stara (Vitis vinifera L.).

Abstract

Somatic mutations in grapes are relatively frequent and associated with diversity in grape skin color and berry morphology. Mutations that occur on a side branch of the a Benitakaa cultivar with rosy-red berry skin color generated the a Brasila cultivar, and mutations that occurred on a side branch of a Brasila generated the a Black Stara cultivar, both showing a black color in the berry skin. Therefore, genetic characterization of the Gret1 retrotransposon and the VvmybA1 gene in a Italiaa, a Rubia, a Benitakaa, a Brasila, and a Black Stara was started to find whether the altered coloration of berries in a Brasila and a Black Stara is a product of different mutation patterns in the investigated sequences. Six primer combinations were used for the amplification of different sequences of the Gret1 retrotransposon and VvmybA1 gene of the five cultivars. Polymerase chain reaction (PCR) of the Gret1 retrotransposon and the VvmybA1 gene and sequencing of the amplified products using six primer combinations showed no different alleles or different nucleotide sequences in a Brasila and a Black Stara. The sequencing of the VvmybA1 gene in the present study showed that the mutations that occurred in the cultivar a Italiaa for generating the a Benitakaa cultivar persisted in the a Brasila and a Black Stara cultivars.