Differential induction of HSP-70 expression in response to IHHNV in white shrimp Litopenaeus vannamei naturally co-infected with IHHNV and IMNV

Abstract

Brazil is becoming one of the main global producers of the shrimp Litopenaeus vannamei. Worldwide outbreaks of viral disease place this aquaculture industry at risk, causing episodic economical loss. The primary viruses for L. vannamei, particularly in northeastern Brazil, are the infectious hypodermal and hematopoietic necrosis virus (IHHNV) and the infectious myonecrosis virus (IMNV). After a period of unusual rainfall, we detected that farmed shrimp developing IMN or IHHN disease were co-infected with both viruses, and the disease outcome resulted from reciprocal IHHNV and IMNV proliferation. To comprehend how the key molecules of innate immunity respond to this double infection, the levels of HSP-70, crustin, penaeidin-3a, and C-type lectin-br1 were assessed by quantitative PCR. HSP-70 expression was expressively up-regulated by IHHNV infection in the gills of double-infected shrimp but not by IMNV infection; the other transcripts were not significantly altered. These findings implicate the HSP-70 as a differential modulator of viral co-infection in shrimp.