Abstract
A prospective study was conducted on 25 Legionella pneumophila culture positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.03). No difference was found concerning the inhibition rate.
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CITATION STYLE
Jaulhac, B., Reyrolle, M., Sodahlon, Y. K., Jarraud, S., Kubina, M., Monteil, H., … Etienne, J. (1998). Comparison of sample preparation methods for detection of Legionella pneumophila in culture-positive bronchoalveolar lavage fluids by PCR. Journal of Clinical Microbiology, 36(7), 2120–2122. https://doi.org/10.1128/jcm.36.7.2120-2122.1998
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