Isolation of plasmids from mycobacteria

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Abstract

A procedure for preparing sodium dodecyl sulfate-salt-cleared lysates of mycobacteria was developed. Standard techniques were employed to demonstrate covalently closed circular DNA in lysates of nine strains of Mycobacterium avium-intracellulare.

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Crawford, J. T., & Bates, J. H. (1979). Isolation of plasmids from mycobacteria. Infection and Immunity, 24(3), 979–981. https://doi.org/10.1128/iai.24.3.979-981.1979

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