Quantification of sunscreen 2-phenylbenzimidazole-5-sulfonic acid in cosmetic products and water samples by HPTLC/densitometry with fluorescent detection

Abstract

The water-soluble sunscreen 2-phenylbenzimidazole-5-sulfonic acid (PBS) was quantified in a sun-care product and water samples by thin layer chromatography followed by densitometric scanning in fluorescence mode (cut-off filter 370 nm, wavelength of excitation - 300 nm). Normal phase TLC was performed on silica gel 60 as stationary phase. Mobile phase used was ethyl acetate-ethanol-water 70:35:30 (v/v/v). The limit of detection (LOD) was 0.0004 μg spot-1, and the limit of quantification (LOQ) was - 0.001 μg spot-1 without any sample pre-concentration. © 2013 Versita Warsaw and Springer-Verlag Wien.