Addition of Coconut Water and Banana Extract on MS Media to Stimulate PLB (Protocorm Like Bodies) Regeneration of Dendrobiumgatton sunray

Abstract

Organic material is widely used for propagation of orchids, namely coconut water, extracts of tomatoes, bananas, bean sprouts and potatoes which can be added to certain media as a provider of nutrients, amino acid minerals, and growth regulators. This study aims to determine the effect of the concentration of organic matter in coconut water and banana extract on MS media on the regeneration of PLB (protocorn like bodies) Dendrobiumgatton sunray. The study was conducted at the Biotechnology Laboratory, Faculty of Agriculture, Bengkulu University, from September to December 2019. This study used a Complete Randomized Block Design (RCBD) with two factors. The first factor was the concentration of coconut water with 3 levels namely: A0 = 0 (Control), A1 = 75 ml.L-1 , A2 = 150 ml.L-1 , and the second factor was the concentration of banana extract with 5 levels namely: B0 = 0 (Control), B1 = 75 g.L-1 , B2 = 150 g.L-1. The results showed that coconut water did not affect all variables, however the banana extract had a significant effect on all variables except for the number of shoots. There was an interaction between the two treatments, namely the variable plantlet weight. Orthogonal polynomial analysis showed that there was an interaction between the two treatments, where coconut water and banana extract had a good response on the plantlet growth when given together as much as 75 ml.L-1 (A1) and 75 g.L-1 (B1). The concentration of both treatment was lower than it was given individually.