Screening, isolation and selection of cellulolytic fungi from cattle rumen fluid for bio-degradator in aquaculture

Abstract

Screening and isolation of cellulolytic fungi was done using cattle rumen fluid conducted at a local slaughtered house in Bandung, west Java, Indonesia. This research was aimed to isolate cellulolytic fungi with over production of cellulase components. Isolated fungi had shown the ability to degrade cellulose base on decolorization of CMC selective agar using Grams iodine as color indicator. Two isolates with cellulolytic activity with the result of CM Case 2.33 and 1.24, and FP-ase 0.30 and 0.11 were identified as P. nalgiovense (SpC.PD) and A. tamarii (SpF.PD). This study identified those species as highly cellulolytic in producing cellulase activity. Identification of the cellulase enzymes can help industries to exploit this enzyme for feed bio-degradator such as in aquaculture production.