Cellular expression of angiotensin type-1 receptor mRNA in the kidney

Abstract

Angiotensin II has multiple renal effects that are important in the regulation of renal hemodynamics and electrolyte secretion, and binding sites for angiotensin II have been demonstrated in different cells of the kidney. In the present study the cellular localization of mRNA for the angiotensin type 1 (AT1) subtype of the angiotensin II receptor was studied in adult rat kidney using a cRNA probe and in situ hybridization. Strong labeling was demonstrated in tubule cells of the inner and outer stripe of the outer medulla. In emulsion-dipped sections, counterstained with hematoxylin-eosin, labeling was identified in segment S3 of proximal tubules and in the thick ascending limb of loop of Henle (mTAL). The results suggest expression of AT1-receptor mRNA with a distinct compartmentalization within the nephron.