The Occurrence of 4‐Amino‐4‐deoxy‐l‐arabinose as a Constituent in Salmonella Lipopolysaccharide Preparations

Abstract

When the lipopolysaccharides of two Salmonella R mutants were hydrolyzed in 0.5 N HC1 at 37° for 7 to 24 h, a previously undescribed sugar was liberated. This sugar has been identified as 4‐amino‐4‐deoxy‐l‐arabinose by comparison of the free amino sugar, its two reduced derivatives, its two N‐acetylated derivatives, and its methyl glycoside with the authentic amino sugar and its corresponding derivatives on thin layer, gas liquid, and column chromatography. A survey of about 50 enterobacterial lipopolysaccharides revealed that this amino sugar is present in a large percentage of the preparations. The preparation of 4‐amino‐4‐deoxyarabinose by the reduction and limited periodate oxidation of 2‐amino‐2‐deoxymannose is described. Copyright © 1970, Wiley Blackwell. All rights reserved