Effect of the aqueous extract of Euphorbia Guyoniana (Euphorbiaceae) on pathogenic bacteria from land-based sources

Abstract

Euphorbia guyoniana is a medicinal plant endemic to Algeria. It is used by local populations for its medicinal properties. This study was to determine the antibacterial effect of the aqueous extract from Euphorbia guyoniana on pathogenic bacteria of telluric origin. The aqueous extract was obtained by confrontation with organic solvents method with a yield of 1.7%. The total content of the flavonoïd extract was evaluated by the method of Aluminum Trichloride and was found to be 0.31 mg EQ/gE. The extract obtained was characterized by Infrared Spectroscopy and revealed a richness of phenol, aldehyde and ester then analyzed by High-Performance Liquid Chromatography, which allowed us to identify 27 flavonoid compounds. The extract was tested by the diffusion method on agar, on 12 bacterial strains isolated from a henhouse and identified by VITEK. These strains seem to be sensitive to the flavonoids of Euphorbia guyoniana with MICs varying from 1.47 to 61.78 mg/ml. The order of sensitivity of the bacterial strains to the extract is represented in the following order: Staphylococcus aureus > Streptococcus faecalis > Escherichia coli. Thus, the flavonoids of Euphorbia guyoniana may be an alternative to chemical control of certain pathogenic microorganisms.