Biochemical analysis by calcium determination on the newly formed bone in critical bone defects submitted to different treatments. A pilot study

Abstract

The aim of this study was to evaluate the calcium dosage of the newly formed bone, in critical bone defects created on the calvarial tissue and filled by different substances, in male Wistar rats. A cylindrical critical bone defect (6x2mm) was created in the posterior portion, left side, on the calvarial bone in nine animals and filled with: animals' coagulum, monoolein gel, poloxamer gel, monoolein gel + 1μg rhBMP-2, monoolein gel + 3μg rhBMP-2, monoolein gel + 7μg rhBMP-2, poloxamer gel + 1μg rhBMP-2, poloxamer gel + 3μg rhBMP-2, poloxamer gel + 7μg rhBMP-2. The results of this study showed a positive correlation between the calcium dosage analysis and the newly formed bone.