Synthesis and characterization of nano-hydroxyapatite in maltodextrin matrix

Abstract

In this study, we report the direct precipitation of nano-HA in the present of maltodextrins with the different dextrose equivalent (DE) values in the range of 10–30. Characterization of the obtained samples, using X-ray diffraction and Fourier transform infrared spectrophotometry, indicated that the presence of maltodextrins, with the different DE values, does not affect the phase composition and structure of the obtained composites. Morphologystudies of the samples, using field emission scanningelectron microscope and transmission electron microscope,revealed that maltodextrin has obvious effect on the size,shape, and morphology of hydroxyapatite nanoparticles. In particular, in studied DE range, maltodextrin DE 28–30 with dominant structure of debranched chain is the most preferable choice to obtain the composite with highly dispersed nanoparticles. In vitro assay on pre-osteoblast MC3T3-E1 cells demonstrated the ability of the composites to stimulate alkaline phosphatase activity and mineralization during differentiation of the cells.