Isolation of mesenchymal stem cells from mice bone marrow (mBMMSCs) from femur and tibia

Abstract

Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into osteocyte, chondrocyte, and adipocyte. MSCs are promising candidates for a regenerative disease therapy because of their ability to self-renewal and multi-lineage differentiation. MSCs can be isolated from bone marrow (BM), adipose tissues, Wharton jelly, placenta, dermis, and dental pulp. The aim of this study was to compare isolation of bone marrow MSCs from femur and tibia of Swiss Webster mice. It was an in vitro laboratory experimental design. Mice BMMSCs were isolated from mice femur and tibia bone marrow with flushing methods. Mice femur and tibia were immersed in povidone-iodine solution and alcohol 70 % to bones decontaminated and removed the attached fat and connective tissue. Both ends of the bone are cut and flushing with culture medium. The cells suspension incubated in 5 % CO2 incubator. Characterization of mBMMSCs was done by observed cells number. Differentiation potency of MSCs into osteocyte, chondrocyte, and adipocyte were tested using the commercially available kit with staining by alizarin red, alcian blue and oil red O. SPSS with independent t-test. The results showed that cells number of mice BMMSCs after first passage from tibia is higher than femur. Both of mice MSCs from femur and tibia can differentiate to osteocyte, chondrocyte, and adipocyte. mBMMSCs can be isolated from femur and tibia with same ability of differentiation to osteocyte, chondrocyte, and adipocyte.