Vernalisation mediated LncRNA-like gene expression in Beta vulgaris

Abstract

Sugar beet (Beta vulgaris L.) cannot form reproductive shoots during the first year of their life cycle. Flowering only occurs if plants are vernalised and are subsequently exposed to long days. However, the vernalisation mechanism remains poorly understood in sugar beet. Three putative lncRNAs associated with vernalisation (AGL15X1, AGL15X2 and CAULIFLOWER A) were investigated and the hypothesis that their expression occurred in response to vernalisation was experimentally tested. The regulation mechanisms of BvRAV1-like, lncRNA-like genes, BvFT1 and BvFT2 were also examined. The BvRAV1-like gene associated with vernalisation in sugar beet was validated for the first time. Our data confirmed the hypothesis that AGLX2 was the first candidate lncRNA of sugar beet and the BvRAV1-like gene was expressed in response to vernalisation. BvRAV1-like and AGLX2 genes might be coordinated with BvFT2 to promote reproductive growth by repressing BvFT1 during cold exposure followed by long day conditions. A new complementary flowering model of sugar beet was proposed. Our findings opened up new possibility for future studies and further illuminated the molecular mechanism of vernalisation in sugar beet.