Random Brownian motion regulates the quantity of human immunodeficiency virus type-1 (HIV-1) attachment and infection to target cell

Abstract

The efficiency of human immunodeficiency virus type-1 (HIV-1) infection in GHOST/CXCR4 cell was controlled by the frequency of virus access, which was limited by random Brownian motion. Prolonged exposure of cell to virus increased the number of infection. Virus migrated to cell, continuously, during exposure period. Virus adsorption amount increased kinetically. It was confirmed by measurement of accumulated provirus DNA sum that the amount of virus entry depended on exposure time. Increment of virus adsorption amount and the number of infection followed to the theoretical manner that virus adsorption dynamics was regulated by random Brownian motion. This observation says that infectivity of lentivirus vector (HIV-1 based) increases by exposure time dependent way. This phenomenon is applicable to enhancing the efficiency of gene transduction by lentivirus vector.