Total Flavonoids from Oroxylum indicum Induce Apoptosis via PI3K/Akt/PTEN Signaling Pathway in Liver Cancer

Abstract

Total flavonoids (TF), derived from the seeds of Oroxylum indicum (L.) Vent., possess many pharmacological functions. In the present study, H 22 ‐bearing mice and SMMC‐7721 models were employed to evaluate the antitumor activity of TF and to and investigate its possible mechanisms both in vitro and in vivo . Cell viability was evaluated by MTT assay; cell apoptosis rate was analyzed via Annexin V‐FITC/PI double staining by flow cytometer. Meanwhile, the expressions of apoptosis‐related mRNA and proteins were evaluated by RT‐PCR and Western blot analysis. The results revealed that TF could significantly inhibit the tumor growth, and the possible mechanism was related to the effect of inducing tumor cells apoptosis through PI3K/Akt/PTEN signaling pathway. This study has provided a theoretical basis for the further development and application of TF as antitumor drugs.