Green fluorescent protein as a reporter to monitor gene expression and food colonization by Aspergillus flavus

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Abstract

Transformants of Aspergillus flavus containing the Aequorea victoria gfp gene fused to a viral promoter or the promoter region and 483 bp of the coding region of A. flavus aflR expressed green fluorescence detectable without a microscope or filters. Expression of green fluorescent protein fluorescence was correlated with resistance to aflatoxin accumulation in five corn genotypes inoculated with these transformants.

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Du, W., Huang, Z., Flaherty, J. E., Wells, K., & Payne, G. A. (1999). Green fluorescent protein as a reporter to monitor gene expression and food colonization by Aspergillus flavus. Applied and Environmental Microbiology, 65(2), 834–836. https://doi.org/10.1128/aem.65.2.834-836.1999

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