First report of chromosome analysis of saddleback anemonefish, amphiprion polymnus (Perciformes, Amphiprioninae), in Thailand

Abstract

We report the first chromosome analysis for the saddleback anemonefish (Amphiprion polymnus) from Thailand. Kidney cell samples were taken from 5 male and 5 female fish. The mitotic chromosome preparation was prepared by directly from kidney cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of A. polymnus was 2n=48 and the fundamental numbers (NF) was 96 in both male and female. The types of chromosomes were 6 large submetacentric, 2 large acrocentric, 10 medium metacentric, 12 medium submetacentric, 6 medium acrocentric, 10 small metacentric, and 2 small submetacentric chromosomes. The region adjacent to the telomeres on the short arms of chromosome pair 21 showed clearly observable secondary constriction/NORs. The karyotype formula for A. polymnus could be deduced as: (Equation presented). © 2012 The Japan Mendel Society.