Potassium increases intracellular calcium simulating progesterone action in human sperm

Abstract

Progesterone (P) and zona pellucida are known to induce acrosome reaction in human sperm by increasing cytosolic calcium. High concentrations of potassium ions (K+) improve the rate of acrosome reaction in human sperm in vitro. This article determined whether the effect of K+ on the acrosome in human sperm is mediated by increasing intracellular calcium ([Ca2+](i)). The effect of K+ on [Ca2+](i) was examined by using Fura 2 as the fluorescent indicator. The effect of K+ and P on [Ca2+](i) in sperm and the involvement of ion channels was compared. Motile sperm were collected by the swim-up method from semen of healthy volunteers and capacitated overnight in BWW containing 0.5% BSA. Incubation of capacitated sperm with different concentrations of potassium chloride (1.25-20 mM) resulted in dose-dependent increase in [Ca2+](i) similar to that observed with P. The increase in [Ca2+](i) by K+ and P was blocked by the addition of EGTA, a Ca2+ chelator. K+-induced change in [Ca2+] was not altered by the addition of dihydropyridine derivatives. The combined treatment of K+ (20 mM) and P (0.75 μg/mL) caused an additive effect on the increase in [Ca2+](i). It would appear that human sperm plasma membrane possess different Ca2+ channels responsive to P and K+.