Genes for iron-sulphur cluster assembly are targets of abiotic stress in rice, Oryza sativa

Abstract

Iron-sulphur (Fe-S) cluster assembly occurs in chloroplasts, mitochondria and cytosol, involving dozens of genes in higher plants. In this study, we have identified 41 putative Fe-S cluster assembly genes in rice (Oryza sativa) genome, and the expression of all genes was verified. To investigate the role of Fe-S cluster assembly as a metabolic pathway, we applied abiotic stresses to rice seedlings and analysed Fe-S cluster assembly gene expression by qRT-PCR. Our data showed that genes for Fe-S cluster assembly in chloroplasts of leaves are particularly sensitive to heavy metal treatments, and that Fe-S cluster assembly genes in roots were up-regulated in response to iron toxicity, oxidative stress and some heavy metal assault. The effect of each stress treatment on the Fe-S cluster assembly machinery demonstrated an unexpected tissue or organelle specificity, suggesting that the physiological relevance of the Fe-S cluster assembly is more complex than thought. Furthermore, our results may reveal potential candidate genes for molecular breeding of rice. Iron-sulfur cluster assembly genes have never been studied in rice, Oryza sativa. This work has the first time identified 41 genes in rice that may be involved in iron-sulfur cluster assembly. We found that these genes specifically responded to abiotic stresses, including heavy metals, iron, and methyl viologen. © 2013 John Wiley & Sons Ltd.