Optimized QuEChERS Methodology for Reliable LC-MS/MS Quantification of Sertraline and Fluoxetine Hydrochloride in Biological Samples

Abstract

In the present study, Liquid chromatography was Coupled with tandem mass spectrometry (LC-MS/MS) coupled with QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe). The development of an analytical technique based on dispersive solid phase extraction (dSPE) has opted for the simultaneous detection of fluoxetine and sertraline hydrochloride at six distinct concentrations in the simulated gastric fluid. The antidepressants were identified and quantified using (ESI+) mode. With limits of detection (LOD) and quantification (LOQ) of 9.42-10.40 ng/ml and 28.15-31.23 ng/ml, respectively, for each analyte, the process was validated. With an R2 > 0.998, the linear calibration curve between 5 to 200 ng/ml was obtained. Moreover, the method exhibited exceptional accuracy and robustness with a recovery range between 70-120% according to SWGTOX guidelines. In addition, this study provides a reliable and efficient approach to extracting and identifying antidepressants from gastric fluid.