Escherichia coli F1Fo-ATP Synthase with a b/δ fusion protein allows analysis of the function of the individual b subunits

Abstract

Background: The essential "stator stalk" connects the nonrotating portions of F1Fo-ATP synthase. Results: A b/δ fusion protein is functional and allows analyzing the role of the individual b subunits. Conclusion: One of the b subunits is required for binding to F 1, the other for binding to Fo. Significance: Understanding the function of the stator stalk is important for understanding the mechanism of ATP synthase. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.