THE EFFECT OF EXTRACTION METHOD OF VERNONIA AMYGDALINA DELILE. LEAVES ON CARDIOTONIC EFFECT

Abstract

Vernonia amygdalina Delile leaves contain secondary metabolites that prevent heart disease. This study aims at demonstrating the cardiotonic effect of various ethanolic extracts obtained with simplicia powder of African leaf. In this direction, we tested the effect of African leaf ethanolic extract on normal rat heart isolates and determined its effect on heart contractility and heart rate. The results showed that the highest cardiac glycoside content in the ethanolic extract obtained by reflux extraction was 30.05 ± 0.13 mg/g. In an inotropic test, the same extract with a concentration of 1 mg/mL increased contractility by 74.02%, and regarding the chronotropic test, an 22.68% increase was observed. African leaf ethanolic extract (from Vernonia amygdalina Delile), obtained using reflux method, with a 1 mg/mL concentration, exhibited positive inotropic and chronotropic activity.