Protective effects and antioxidant mechanism of bamboo leaf flavonoids on hepatocytes injured by CCl4

Abstract

The protective effects and antioxidant mechanisms of bamboo leaf flavonoids (BLFs) on hepatocytes injured by CCl4 were studied by establishing models of hepatocyte damage induced by CCl4 and detecting the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutathione peroxidase (GSH-Px), superoxide dismurase (SOD), malondialdehyde (MDA) content and liver glycogen content. The degree of lesions in liver tissues between model and dosage groups was observed through paraffin sections. Results showed that BLFs significantly inhibit CCl4-induced liver injury. The ALT and AST activities as well as MDA contents of cells decreased significantly after treatment with BLFs. GSH-Px and SOD activities as well as liver glycogen contents remarkably increased in the flavonoids groups, which exhibited dose-dependent relationships (P < 0.05). The degree of lesions in liver tissues in the BLF groups was microscopically better than that in the model group. BLFs also significantly suppressed hepatocellular injury and death of apoptotic cells. Therefore, BLFs have remarkable protective effects on acute liver injury, which is related to its strong antioxidant capacity to reduce damage in the liver caused by oxidative stress and cell (NCTC-1469) apoptosis. The results of this study provide pharmacological evidence to support the clinical application of BLFs. © 2013 Taylor & Francis.