Evaluation of bacterial and archaeal diversity in the rumen of Xiangxi yellow cattle (Bos taurus) fed Miscanthus sinensis or common mixed feedstuff

Abstract

Miscanthus sinensis is a potential biofuel that is distributed widely in China, but with difficulties for decomposition and utilization due to the complexity of its fibrous cell walls. To detect whether M. sinensis could increase the population of rumen fibrolitic microbes, two16S rRNA gene libraries were constructed using ruminal samples from Xiangxi yellow cattle fed with either common mixed feedstuff (group C) or M. sinensis (group M), and the diversity of ruminal bacteria and archaea in the rumens of cattle of both groups was identified. Based on the comparative analysis of these two groups, the microbial composition in group C/M was found to be: Bacteroidetes (16.33 %/28.15 %), Firmicutes (68.88 %/60.92 %), Proteobacteria (10.71 %/3.78 %), Planctomycetes (0/0.84 %), Lentisphaerae (0/0.42 %), Spirochaetes (1.02 %/0) in the Bacteria domain and Thermoplasmata (13.09 %/46.67 %), Methanomicrobia (57.14 %/12.22 %) and uncultured archaea (29.76 %/41.11 %) in the Archaea domain, respectively. Moreover, through phylogenetic analysis, we also detected the increase of Bacteroidetes and the decrease of Methanomicrobia in group M. These results indicated that feeding cattle with M. sinensis will change the microbial composition in the rumen; the increased bacteroidetes may be responsible for digesting M. sinensis, which will benefit us in further screening for potentially valuable bio-enzymes. © 2013 Springer-Verlag Berlin Heidelberg and the University of Milan.