Hybridization and breeding of the benomyl resistant mutant, trichoderma harzianum antagonized to phytopathogenic fungi by protoplast fusion

Abstract

A diploid strain obtained from heterokaryons of Trichoderma harzianum by protoplast fusion grew on minimal medium containing 100 ppm benomyl. This strain inhibited the growth of the phytopathogenic fungus Fusarium oxysporum f. sp. raphani on paired cultures and also protected against radish yellows and a drop in germination induced by F. oxysporum f. sp. raphani. © 2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry.