Distribution of Geriatric Disease-Related Genotypes in the National Institute for Longevity Sciences, Longitudinal Study of Aging (NILS-LSA)

Abstract

Phenotypes of various genes related to geriatric diseases and the aging process were assessed in the National Institute for Longevity Sciences, Longitudinal Study of Aging (NILSLSA). The subjects were 1,297 participants in the NILS-LSA. They were community-living males and females aged 40 to 79 years who were randomly selected from the area of the NILS. Genotypic and allelic frequencies of genes in the subjects were analyzed. Age and gender differences in the distribution of genotypes were also tested. The genotypic frequencies were as follows: (1) Angiotensin l-converting enzyme (ACE) genotype was I/I 46.2%, I/D 38.3% and DID 15.5%. (2) a 1-adrenoreceptor genotype was C/C 84.4%, C/T 12.7%, and TIT 3.0%. (3) Apolipoprotein E genotype was s 21 e 2 0%, £21 £3 7.9%, s 31 £3 70.0%, £31 £4 20.8%, £21 £4 0%, and £41£4 1.4%. (4) Cholecystokinin type-A receptor (CCKAR) nucleotide -81 (nt-81) genotype was A/A 59.1 %, A/G 35.1 %, and G/G 5.9%. The CCKAR nucleotide -128 genotype (nt128) was GIG 74.3%, G/T 23.6%, and T/T 2.2%. The combination of nucleotide (nt-81, nt-128) was (AIA, G/G) 59.1%, (A/G, G/G) 14.1%, (G/G, G/G) 1.1%, (A/G, G/T) 21.0%, (G/G, G/T) 2.6%, and (G/G, T/T) 2.1%. There were no subjects with (A/A, GIT), (A/A, T/T) or (A/G, T/T) genotypic combinations. (5) ,3 3-adrenoreceptor genotype was T/T 66.8%, T/A 28.5%, and A/A 4.7%. (6) Dihydrolipoamide succinyltransferase (DLST) nucleotide 19117 genotype was A/A 25.1%, A/G 49.7%, and G/G 25.1%. The DLST nucleotide 19183 genotype was C/C 55.8%, C/738.2%, and 7/75.9%. The combination of nucleotide (nt19117, nt19183) was (A/A, C/C) 6.7%, (A/G, C/C) 24.1%, (G/G, C/C) 25.1%, (A/G, C/T) 25.6%, (A/A, 7/7) 5.9%, and (A/A, C/T) 12.6%. There were no subjects with (A/G, 7/7), (G/G, 7/7) or (G/G, 7/C) genotypic combinations. (7) Transforming growth factor-,91 genotype 7/735.2%, 7/C 44.6%, and C/C 20.2%. (8) The platelet-activating factor acethylhydrase genotype was M/M 71.7%, M/m 27.2%, and m/m 1.2%. The mitochondria DNA 5178 genotype A was 42.1% and C was 57.9%. There were no significant gender or age differences in tested genotypic and allelic distribution except for the DLST and apolipoprotein E. Differences in the genotypic frequencies of distribution using the Ha'rdy-Weinberg equilibrium were siqnificant in the ACE and a 1 -adrenoreceptor genotypes.