The antibacterial inhibition test of gonad sea urchin (diadema setosum) against the growth of staphylococcus aureus

Abstract

A The sea urchin is sea animal the rich in vitamin A, vitamin B complex and minerals to the smooth functioning of the nervous system and the metabolism of the human body. Gonad sea urchin contains flavonoids, steroids, triterpenoids, and saponins. This research was conducted to determine inhibition of antibacterial ointment sea urchin gonads against Staphylococcus aureus. The type of research conducted is experimental. This research used a cylinder cup method to measure inhibition zone of gonad sea urchin gonad. After incubation for 24 hours, measured inhibitory zone diameter around the cylinder cup. The results showed that the sea urchin gonads ointment have inhibitory effect on the growth of Staphylococcus aureus bacteria with broad average inhibition zone produced at a concentration of 2% at 2.47 mm, 4% of 1.62 mm, and a concentration of 6% has inhibitory zone Of 1.12 mm, and a positive control of 11.55 mm and the negative control does not provide a drag zone. Based on the ANOVA test results can be concluded that the F test = 0.691