The effect of methyl jasmonate and phenolic acids on growth of seedlings and accumulation of anthocyanins in common buckwheat (Fagopyrum esculentum Moench)

Abstract

The effect of methyl jasmonate (JA-Me) and phenolic acids: trans -cinnamic acid ( t -CA), p -coumaric acid ( p -CA), salicylic acid (SA) as well as naringenine (NAR) on growth of seedlings and accumulation of anthocyanins in common buckwheat ( Fagopyrum esculentum Moench) were studied. JA-Me and phenolics were applied to growth medium of 4-days etiolated buckwheat seedlings before their exposition to day/night (16h/8h) conditions. The increase of primary roots and hypocotyls length were measured after 3 days of seedling growth in such conditions. At the end of experiment the total anthocyanins contents were measured as well. Methyl jasmonate (JA-Me) and trans-cinnamic acid ( t -CA) inhibited growth of the primary root in young buckwheat seedlings, while naringenine (NAR) had a stimulatory influence, and p -coumaric acid had no effect at all. None of investigated phenolics or JA-Me had an effect on the growth of buckwheat hypocotyls, except the mixture of JA-Me and p -coumarcic acid. JA-Me significantly decreased the anthocyanins level in buckwheat hypocototyls, but not in cotyledons. trans -Cinnamic acid, p -coumaric acid and naringenine had no significant influence on the anthocyanin level in hypocotyls and cotyledons of buckwheat seedlings. Simultaneous treatment of buckwheat seedlings with JA-Me and t -CA or p -CA did not change the inhibition of anthocyanins accumulation in buckwheat hypocotyls by JA-Me. In the hypocotyls of buckwheat treated with a mixture of JA-Me and NAR, or SA, a synergistic reduction of anthocyanins was observed.