Continuous measurement of retinoic acid isomers and retinol in a plasma by column-switching reversed-phase HPLC

Abstract

A specific and sensitive method for the determination of three retinoic acid isomers (13-cis-retinoic acid, all-trans -retinoic acid and 9-cis-retinoic acid) and retinol using column- switching, HPLC was developed. The three acids and retinol were completely separated within 25 min. The detection limit was ca. 20 pg for the three acids, respectively. Linearity was obtained up to 200 ng for all three acids. The relative standard deviation (n=10, μg/ml) of a within run was 0.5~1.9% for all three acids. This method was applied to the determination of three acids and retinol in human plasma following oral administration with 13-cis-retinoic acid. © 1997, The Japan Society for Analytical Chemistry. All rights reserved.