Molecular cloning, characterization and differential expression of a Sporothrix schenckii STE20-like protein kinase SsSte20

Abstract

Dimorphic switching requires fungal cells to undergo changes in polarized growth in response to environmental stimuli. The Ste20-related kinases are involved in signaling through mitogen-activated protein kinase pathways and in morphogenesis through the regulation of cytokinesis and actin-dependent polarized growth. In this report, we isolated and characterized an Ste20 homologue gene, designated SsSte20, from yeast-form Sporothrix schenckii (S. schenckii). The full length SsSte20 cDNA sequence is 2846 bp in size, and contains an open reading frame of 2505 bp encoding 835 amino acids. The predicted molecular mass of SsSte20 is 91.31 kDa with an estimated theoretical isoelectric point of 5.76. SsSte20 kinase domain shows 63% identity with that of Don3, a germinal centre kinase (GCK) from Ustilago maydis. Two exons and one intron are identified within the 2578 bp SsSte20 genomic DNA sequence of S. schenckii. Differential expression of the SsSte20 was demonstrated by real-time RT-PCR. The expression of SsSte20 was much higher in the yeast stage compared with that in the mycelial stage, which indicated that the SsSte20 may be involved in the pathogenesis of the yeast phase of S. schenckii.