Development and validation of area under curve spectrophotometry method for ternary mixture of dextromethorphan HBr, doxylamine succinate and pseudoephedrine HCl in tablet dosage form

Abstract

This study aimed to develop spectrophotometry method using area under curve to determine the levels of dextromethorphan HBr (DMP), doxylamine succinate (DOX) and pseudoephedrine HCl (PSE) in tablet dosage form using ethanol as solvent. The method is based on the AUC method absorption measurement in the wavelength ranges 273-283 nm for DMP, 262-272 nm for DOX and 251-261 nm for PSE. Then, the method was applied to determine the levels of DMP, DOX and PSE in tablet dosage form. The selection of wavelengths based on wavelengths gives the best result. The mean % recoveries were found to be in 100.89%, 100.52%, and 100.69% for DMP, DOX and PSE, respectively. The method is successfully applied to analyze DMP, DOX and PSE in pharmaceutical formulation with no interference from excipients as indicated by the recovery study. All validation parameters were within the acceptable range.