Antibacterial Activity of Kitolod (Isotoma Longiflora L.) Herb Extract and Fractions Against the Growth of Dental Carises Causing Bacteria Streptococcus Mutans

Abstract

The Kitolod herb (Isotoma longiflora L.) has empirically been used to treat several diseases. Kitolod herb contains some secondary metabolite compounds, such as flavonoids, tannins, and saponins, which are antibacterial. This study aims to determine the inhibition of antibacterial activity and the most effective concentration in inhibiting Streptococcus mutans bacteria. The sample used in this study was Kitolod herb obtained from Sentani Regency. Then, the sample was extracted using the maceration method with 96% ethanol solvent. Then, it was fractionated using the solvent of ethanol-water, ethyl-acetate, and n-hexane. The antibacterial activity testing used the disc diffusion method. This study used a completely randomized design with nine treatments that included the concentrations of 50 ppm, 100 ppm, 200 ppm, 400 ppm, 600 ppm, 800 ppm, 1000 ppm, amoxicillin (positive control), sterile distilled water (negative control). The results showed that the extract of Kitolod herb has activity as an antibacterial on Streptococcus mutans at the highest concentration of 1000 ppm of 10.20 mm (strong category). The testing results on the three fractions showed that the ethyl-acetate fraction has the most significant inhibition activity of 8,16 mm at a concentration of 1000 ppm (medium category). The result showed that the extract and fraction of Kitolod herb (Isotoma longiflora L.) have the potential to be an antibacterial to inhibit Streptococcus mutans bacteria.