Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay

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Abstract

A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species. Copyright © 2011, American Society for Microbiology. All Rights Reserved.

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Choi, G. E., Chang, C. L., Whang, J., Kim, H. J., Kwon, O. J., Koh, W. J., & Shin, S. J. (2011). Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay. Journal of Clinical Microbiology, 49(3), 1107–1109. https://doi.org/10.1128/JCM.02318-10

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