Nematicidal effects of Leucaena leucocephala and Gliricidia sepium extracts on Meloidogyne incognita infecting okra

Abstract

Two separate but identical greenhouse trials were conducted to investigate the effects of extracts of leaves and roots of Leucaena leucocephala and Gliricidia sepium each at 80,000 mg / kg and 40,000 mg / kg on Meloidogyne incognita on okra. Each potted nine-day old okra seedling was inoculated with 3,000 fresh eggs of M. incognita and 5ml of each of the extracts was added simultaneously around the roots of the seedling. Treatment of okra plant with L. leucocephala and G. sepium extracts resulted in reduced nematode population, reduced galling, reduced nematode reproduction rate and enhanced fruit weight. Chemical analysis revealed that G. sepium leaves contained phenolic compound and carboxylic acid, while the roots showed the presence of aromatic amide, phenolic compound and carboxylic acid. L. leucocephala leaves showed the presence of phenolic compound, aromatic amide and carboxylic acid, while the roots showed the presence of phenolic compound and carboxylic acid. The results of this study suggest that leaf and root extracts of L. leucocephala and G. sepium at the rate of 40,000 mg / kg could be useful in root knot nematode management in vegetable beds.Delovanje ekstraktata listova i korena Leucaeana leucocephala i Glicidia sepium na fitoparazitne korenove nematode Meloidogyne incognita u proizvodnji bamije, ispitivano je u Nigeriji u saksijskim ogledima u kontrolisanim staklenickim uslovima. Sejanci su inokulisani sa po 3000 jaja nematode, i potom je dodavano po 5 ml biljnog ekstrakta. Tretman ispitivanim ekstraktima rezultirao je, u odnosu na kontrolu, smanjenom populacijom nematode, manjim i vecim prinosom plodova bamije. Hemijska analiza utvrdila je fenole i karboksilnu kiselinu u ekstraktima listova i korena G. sepium, a u listovima L. leucocephala jos i aromaticne amide. Rezultati ukazuju da ispitivani ekstrakti imaju potencijal za suzbijanje M. incognita.