Effectiveness of chitosan from crab shell as antibiotic for Escherichia coli

Abstract

Background: Research in finding a safe, effective and natural ingredient against Escherichia coli continues to be carried out as a result of an increasing antibiotic resistance. Chitosan has been known as a food preservative but the research for its effectivity against Eschericia coli is still inadequate. Indonesia is rich of natural animal resources including crustaceans, like Crab. Objective : How effective are chitosans extracted from crab shells as an antibiotic against Escherichia coli when compared with ceftriaxone. Method : Experimental method with a static group comparison where E. Coli in Mueller Hinton Agar (MHA) on petri dishes was given chitosan intervention with initiating concentrations starting from 4% to 8% and 30mcg of ceftriaxone given by diffusion. The antimicrobial of E.coli was measured with Whatman paper and standard calipers measured in millimeters. Result : Results of normality and homogeneity variance tests with (p > 0,05) were inhomogenous and not distributed normally so it was analyzed with Kruskal Wallis Test continued with a Mann Whitney Test. Conclusion : Chitosan solution extracted from crab shells with concentrations of 4%, 5%, and 6% have an antibiotic effect against the growth of E.coli. The most effective concentration to inhibit E.coli is 4%.