Isolation and identification of the causative agent of bacterial throat infection according to a response to commonly antibiotic

Abstract

Bacterial throat infection has a worldwide distribution, affecting persons of all ages, races, social strata and both genders. The present study was a prospective cross-sectional study aimed to isolate and identify the bacteria which cause throat infection and their antibiotic susceptibility in Madani – Sudan. Throat swabs were collected from 100 patients attended to Ear, Nose and Throat (ENT) Teaching Hospital. Isolation and identification of bacteria were made by directly inoculated onto blood agar, Gram stain and biochemical test. All isolated organisms were tested for their in vitro antimicrobial susceptibility against various antibiotics using the Kirby-Baur disk diffusion method. The major organism isolated was S. aureus (60%), and the least organism isolated was S. pneumonia (6.7%). The maximum antibiotic sensitivity of the isolates was against were erythromycin, ceftriaxone, cefuroxime, and ciprofloxacin. In This study, most the samples are negative for bacterial infections. S. aureus is the most common causes of throat infections and resists to antibiotics compared to other bacteria showed no resistance to all antibiotics.