Engineering of a cytidine 5′-Monophosphate-sialic acid synthetase for improved tolerance to functional sialic acids

Abstract

Sialic acid-containing glycoconjugates at the cell surface are of high importance in carbohydrate-mediated recognition phenomena in physiological and pathological events, as well as in bacterial or viral infection. A key step in the enzymatic synthesis of natural sialoconjugates and functional synthetic analogues is the activation of sialic acids to cytidine 5′-monophosphate (CMP)-sialic a Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.