Protective efficiency of parsley (Petroselinum crispum) against oxidative stress, DNA damage and nephrotoxicity induced with anti- tuberculosis drugs

Abstract

Background: Tuberculosis has been seen worldwide as a serious disease, one of the most adverse side effects of main anti-tuberculosis drugs is nephrotoxicity. Aim: the purpose of this research was planned To investigate the nephroprotective ability of aqueous parsley extract against Isoniazid® and Rifampicin®-induced nephrotoxicity. Materials and Methods: Adult male Wistar albino rats weighted (140-160) and they were divided randomly into four groups: 1) normal rats as a control group, 2) rats administrated with parsley extract (250 mg/kg/day), 3) rats received Isoniazid® and Rifampicin® (50 &100 mg/kg/day), and 4) rats treated with Isoniazid® and Rifampicin® In association with the extract of parsley. Results: The results showed, after six weeks, that parsley extract minimized the Isoniazid® and Rifampicin®- induced renal deterioration; This was shown by a significant decrease in serum levels of urea, creatinine, uric acid, TNF-α, IL-1β and Na+ as well as kidney MDA, nitric oxide and DNA damages. This was coupled with a significant improvement in serum calcium and K+ levels and kidney GSH and SOD activity. In addition, the histopathological results indicated that the extract succeeded in the prevention of Isoniazid® and Rifampicin® induced tissue degenerations. Conclusion: In conclusion, parsley extract may be as promising as nephroprotection against Isoniazid® and Rifampicin® nephrotoxicity through their antioxidant and radical scavenging activities.