Potential for Crude Oil and Diesel Biodegradation by the Indigenous Pseudomonas sp. Strain LGMS7 Using GC-MS and GC-FID Analyses

Abstract

Pollution caused by hydrocarbons in the environment is a serious global issue, especially in countries in development such as Algeria. In this context, Pseudomonas sp. strain LGMS7 (MT071345) previously isolated from a soil contaminated with hydrocarbons, in Algeria, was selected to evaluate its crude oil and diesel degradation capacity, individually. For this, we have used an MSM medium with 1% (v/v) of crude oil and 2% (v/v) of diesel as the sole carbon sources, individually, incubated for 27 days at room temperature (25 ± 1 °C) and 150 rpm, and analysed with GC-MS and GC-FID, respectively. Consequently, after analysing of the chromatogram, the results revealed the presence of a linear fraction of aliphatic hydrocarbons n-alkane (C13-C30) of average molecular weight for crude oil, with highly biodegradation efficiency of 98.50%. Nevertheless, biodegradation efficiency of 100% was recorded for the lightest molecules of the n-alkane fraction (C13-C30), which corresponds to the C13-C14 molecules, and for the heaviest molecules of the same n-alkane fraction, which corresponds to molecules C27-C30. Furthermore, biodegradation efficiency >96.00% was recorded for the C15-C26 fraction. While for diesel, biodegradation efficiency of 70% was recorded. Because of its intriguing biodegradation properties for hydrocarbons, this strain appears to be a promising bioremediation candidate for hydrocarbon-polluted soils in Algeria.