Molecular and Morphological Analyses Support Different Taxonomic Units for Asian and Australo-Pacific Forms of Ischnura aurora (Odonata, Coenagrionidae)

Abstract

Despite the great technological progress that has aided taxonomical identification, taxonomical issues remain for certain species found in remote and/or understudied geographical areas. The damselfly species Ischnura aurora has been the subject of a long-standing taxonomical debate, focused mainly on the existence of morphological and behavioural differences between Asian and Australo-Pacific forms of this species that could justify their placement into two different species. Here, we carried out a comparative morphological analysis of specimens currently identified as I. rubilio from India and I. aurora from Asia and Oceania, combined with the analysis of mitochondrial and nuclear sequence data, both developed by us and available in public repositories. Our results split the Asian and Australo-Pacific forms of I. aurora into two well-differentiated taxonomic units and, hence, different (albeit closely related) species, and support the specific status of I. rubilio. The results of our genetic analyses suggest the existence of a third (and even fourth) taxonomic unit, stressing the need to revise all available material belonging to the different I. aurora subspecies that have been described. Finally, we have identified several questionable DNA sequences currently available in public repositories, upon which previous conclusions about the phylogenetic position of I. rubilio are based. Our study stresses the importance of being able to link available DNA sequence data with voucher specimens as well as to carry out a careful examination of DNA sequence data prior to their inclusion in taxonomical studies.